A label-free differential quantitative proteomics analysis of a TaLEA-introduced transgenic Populus simonii × Populus nigra dwarf mutant.
Identifieur interne : 002C23 ( Main/Exploration ); précédent : 002C22; suivant : 002C24A label-free differential quantitative proteomics analysis of a TaLEA-introduced transgenic Populus simonii × Populus nigra dwarf mutant.
Auteurs : Su Chen [République populaire de Chine] ; Hong-Mei Yuan ; Gui-Feng Liu ; Hui-Yu Li ; Jing JiangSource :
- Molecular biology reports [ 1573-4978 ] ; 2012.
Descripteurs français
- KwdFr :
- Phénotype (MeSH), Populus (croissance et développement), Populus (génétique), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéomique (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Tamaricaceae (génétique), Techniques de transfert de gènes (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus, Végétaux génétiquement modifiés.
- génétique : Populus, Protéines végétales, Tamaricaceae, Végétaux génétiquement modifiés.
- métabolisme : Protéines végétales, Végétaux génétiquement modifiés.
- Phénotype, Protéomique, Régulation de l'expression des gènes végétaux, Techniques de transfert de gènes.
English descriptors
- KwdEn :
- Gene Expression Regulation, Plant (MeSH), Gene Transfer Techniques (MeSH), Phenotype (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Proteomics (MeSH), Tamaricaceae (genetics).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- genetics : Plants, Genetically Modified, Populus, Tamaricaceae.
- growth & development : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified.
- Gene Expression Regulation, Plant, Gene Transfer Techniques, Phenotype, Proteomics.
Abstract
TaLEA (Tamarix androssowii late embryogenesis abundant gene, DQ663481) gene was introduced into Populus simonii × Populus nigra by Agrobacterium tumefaciens-mediated transformation with the aim of improving salt-tolerance. Among the 15 transgenic lines, one showed a dwarf phenotype (dwf1). Under the same growth conditions, dwf1 height was significantly reduced compared with the wild-type and the other transgenic lines. The mechanisms underlying this effect were investigated in mutant and wild-type plants using a label-free quantitative proteomics approach. Among proteins that identified, 99 were significantly altered. With the exception of proteins with unidentified or unclassified functions, these proteins were classified into eight groups based on gene product subcellular localization and biological process (metabolism, stress, protein synthesis and degradation, transcriptional regulation, cell fate, transportation, cell wall, and cytoskeleton). Differential expression patterns were identified for key enzymes involved in major metabolic pathways such as the Calvin cycle and glycolysis, thus indicating the interplay of complex molecular events in generation of the dwf1 mutant. Overall, the differentially expressed proteins in dwf1 might provide some useful insights into the dwarf formation.
DOI: 10.1007/s11033-012-1600-5
PubMed: 22327784
Affiliations:
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G" first="Gui-Feng" last="Liu">Gui-Feng Liu</name></author><author><name sortKey="Li, Hui Yu" sort="Li, Hui Yu" uniqKey="Li H" first="Hui-Yu" last="Li">Hui-Yu Li</name></author><author><name sortKey="Jiang, Jing" sort="Jiang, Jing" uniqKey="Jiang J" first="Jing" last="Jiang">Jing Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22327784</idno><idno type="pmid">22327784</idno><idno type="doi">10.1007/s11033-012-1600-5</idno><idno type="wicri:Area/Main/Corpus">002B38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002B38</idno><idno type="wicri:Area/Main/Curation">002B38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002B38</idno><idno type="wicri:Area/Main/Exploration">002B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A label-free differential quantitative proteomics analysis of a TaLEA-introduced transgenic Populus simonii × Populus nigra dwarf mutant.</title><author><name sortKey="Chen, Su" sort="Chen, Su" uniqKey="Chen S" first="Su" last="Chen">Su Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Hong Mei" sort="Yuan, Hong Mei" uniqKey="Yuan H" first="Hong-Mei" last="Yuan">Hong-Mei Yuan</name></author><author><name sortKey="Liu, Gui Feng" sort="Liu, Gui Feng" uniqKey="Liu G" first="Gui-Feng" last="Liu">Gui-Feng Liu</name></author><author><name sortKey="Li, Hui Yu" sort="Li, Hui Yu" uniqKey="Li H" first="Hui-Yu" last="Li">Hui-Yu Li</name></author><author><name sortKey="Jiang, Jing" sort="Jiang, Jing" uniqKey="Jiang J" first="Jing" last="Jiang">Jing Jiang</name></author></analytic><series><title level="j">Molecular biology reports</title><idno type="eISSN">1573-4978</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Transfer Techniques (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Proteomics (MeSH)</term><term>Tamaricaceae (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Phénotype (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Tamaricaceae (génétique)</term><term>Techniques de transfert de gènes (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Tamaricaceae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term><term>Tamaricaceae</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Gene Transfer Techniques</term><term>Phenotype</term><term>Proteomics</term></keywords><keywords scheme="MESH" 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Among the 15 transgenic lines, one showed a dwarf phenotype (dwf1). Under the same growth conditions, dwf1 height was significantly reduced compared with the wild-type and the other transgenic lines. The mechanisms underlying this effect were investigated in mutant and wild-type plants using a label-free quantitative proteomics approach. Among proteins that identified, 99 were significantly altered. With the exception of proteins with unidentified or unclassified functions, these proteins were classified into eight groups based on gene product subcellular localization and biological process (metabolism, stress, protein synthesis and degradation, transcriptional regulation, cell fate, transportation, cell wall, and cytoskeleton). Differential expression patterns were identified for key enzymes involved in major metabolic pathways such as the Calvin cycle and glycolysis, thus indicating the interplay of complex molecular events in generation of the dwf1 mutant. Overall, the differentially expressed proteins in dwf1 might provide some useful insights into the dwarf formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22327784</PMID><DateCompleted><Year>2012</Year><Month>11</Month><Day>23</Day></DateCompleted><DateRevised><Year>2012</Year><Month>05</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-4978</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>7</Issue><PubDate><Year>2012</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Molecular biology reports</Title><ISOAbbreviation>Mol Biol Rep</ISOAbbreviation></Journal><ArticleTitle>A label-free differential quantitative proteomics analysis of a TaLEA-introduced transgenic Populus simonii × Populus nigra dwarf mutant.</ArticleTitle><Pagination><MedlinePgn>7657-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11033-012-1600-5</ELocationID><Abstract><AbstractText>TaLEA (Tamarix androssowii late embryogenesis abundant gene, DQ663481) gene was introduced into Populus simonii × Populus nigra by Agrobacterium tumefaciens-mediated transformation with the aim of improving salt-tolerance. Among the 15 transgenic lines, one showed a dwarf phenotype (dwf1). Under the same growth conditions, dwf1 height was significantly reduced compared with the wild-type and the other transgenic lines. The mechanisms underlying this effect were investigated in mutant and wild-type plants using a label-free quantitative proteomics approach. Among proteins that identified, 99 were significantly altered. With the exception of proteins with unidentified or unclassified functions, these proteins were classified into eight groups based on gene product subcellular localization and biological process (metabolism, stress, protein synthesis and degradation, transcriptional regulation, cell fate, transportation, cell wall, and cytoskeleton). Differential expression patterns were identified for key enzymes involved in major metabolic pathways such as the Calvin cycle and glycolysis, thus indicating the interplay of complex molecular events in generation of the dwf1 mutant. 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